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Objective To evaluate the role of CCAAT/enhancer-binding protein homologous protein (CHOP) in ischemia-reperfusion induced lung injury, and clarify the potential molecular mechanism.Methods Fifty mice of C57BL/6J were randomly divided into five groups, 10 mice in each group, including Sham operation group(sham group),ischemia/reperfusion group (I/R group), I/R +PBS+Lipofectamine group(I/R+PBS+Lipo group) , I/R+scramble siRNA group( I/R+siRNASCR group) , I/R+CHOPsiRNA group( I/R+siRNACHOP group).The content of total lung water (TLW), wet-to-dry weight ratio (W/D) of lung and index of quantitative assessment of histologic lung injury (IQA) were detected, CHOP mRNA and protein expression were detected by RT-PCR and Western blot.Results Compared with Sham group, W/D, TLW and IQA were significantly elevated in I/R group,I/R+PBS+Lipo group and I/R+siRNASCR group (P<0.05).Moreover, the W/D,TLW and IQA reduced with CHOP-siRNA treatment, respectively(P<0.01).Compared with Sham group, CHOP mRNA and protein expressions were significantly elevated in I/R group,I/R+PBS+Lipo group and I/R+siRNASCR group, Moreover, the CHOP mRNA and protein expressions reduced with CHOP-siRNA treatment, respectively(P<0.01). Conclusion I/R could cause excessive unfolded protein response in lung tissue, and induce apoptosis by CHOP signal pathway, damage lung tissue. The inhibition of CHOP pathway could alleviate lung ischemia-reperfusion injury.
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Objective To observe the clinical effect of therapy of budesonide/formoterol powder for inhalation in treatment of adult with bronchial asthma.Methods 90 cases with bronchial asthma and were divided into observation group and control group randomly from February 2014 to February 2015.45 cases in each group.Control group was treated with budesonide inhalants +formoterol inhalants, observation group was given budesonide/formoterol powder inhalation.Changes of related indicators were followed up and recorded.Results After treatment, IL-5, IL-12 and IFN-γwere (60.2 ±9.7)pg/mL,(31.4 ±3.1)pg/mL,(1.6 ±0.2) ng/mL of observation group were better than control group (72.8 ±10.7)pg/mL,(38.5 ± 5.6)pg/mL,(2.3 ±0.3) ng/mL (P<0.05).After treatment,total effective rate in observation group was 68.9%, which was better than control group (48.9%)(P<0.05).Conclusion Clinical effect of budesonide/formoterol powder for inhalation in treatment of patients with bronchial asthma is accurate, and there is no obvious adverse reaction.
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Objective To observe the effect and safety of pleural effusion treated by closed thoracic drainage with micro-catheter. Methods A total of 64 cases with pleural effusion were randomly divided into conventional therapy group ( control group ) and closed thoracic drainage with micro-catheter group ( treatment group) , each group including( 32 cases ). Control group were treated with drainage of thoracic puncture interruptedly while treatment group treated by closed thoracic drainage with micro-catheter. Results Both two therapeutic methods could reduce pleural effusion, but obvious effective rate of treatment group was higher than that of control group( P ＜ 0.05 ). Meanwhile, the rate of pleural reaction and treatment cost in treatment group were lower than that in control group. Conclusion Closed thoracic drainage with micro-catheter was effective, lower treatment cost and lower complications. Moreover, it could reduce medical risk.
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Objective To observe the efficiency of two nebulization inhalation therapy methods in patients with non-invasive ventilation. Methods 60 patients with non-invasive ventilation were divided in-to two groups (30 cases in each group). Based on combination therapy, the observation group received oxy- gen drived nebulizer inhalation therapy through tube system of non - invasive ventilator, the control group re- ceived inhalation therapy by oxygen drived nebulizer directly with halt of non- invasive ventilation. The effi- ciency of two methods was compared between the two groups. Results At the end of treatment SaO2 in the observation group remained nearly unchanged, while that of the control group significantly de-creased,beart rate increased in both groups,bot the amplitude was lower in the observation group. Patients in the observation group showed no evident discomfort, alleviated gasp and eosy expectoration. Conclu-sions Inhalation therapy through tube system of non- invasive ventilator was more efficient and it is worthy of wide clinical application.
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Objective To evaluate the diagnostic value of serum neuron specific enolase(NSE) dynamic changes in brain death patients.Methods After onset of brain death,the serum NSE levels at 24,48 and 72 hours were determined by enzyme linked immunosorbent assay(ELISA) in 30 such patients(brain death group),and the NSE levels were also determined by the same method in 30 extra severe craniocerebral injury patients(cerebral injury group)as concurrent control for comparisons.Results The serum NSE level increased over time both in brain death and severe craniocerebral injury patients.The serum NSE levels of brain death patients were higher than those in extra severe craniocerebral injury patients significantly at 24,48 and 72 hours after onset respectively(all P370 ?g/L) in 5 days,and 3 cases in 7 days.But similar phenomenon was not observed in 13 cases with severe craniocerebral injury and having stayed in ICU over 1 week whose serum NSE level,on the contrary,was decreased gradually after 3 days of onset. It was statistical significant when the serum NSE levels were compared at the same time points between the two groups(P